Chromatography Lab Report Assignment

Chromatography Lab Report Assignment Words: 575

Visible light is a form of electromagnetic radiation. The way we see colors depends on each wavelength and how it interacts with molecules and structures in our eyes. Specific wavelengths have energy that belongs to gaps between electron energy levels in atom. When light is absorbed, electrons move to a higher energy level whereas when they’re released, they move back down to a lower energy level. We used chromatography to separate the colors in the solution and once they were separated, the visible spectroscopy was used to measure the light that went through solution.

The machine contains a bulb which gives visible light, using a prism; the machine selects a wavelength to analyze. Based on the results of this lab, the components that made up the Cool-Aid were separated successfully and well done. Materials and Methods: Before starting anything, it is important to put your safety goggles on. There were two main parts to this experiment. The first one is the chromatography part where you start by filling the syringe with 70 percent of sopranos solution. After attaching a column to it, you continue by running 10 ml of the solution through it.

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An important part to this is making sure the liquids go one direction. After removing the column, you repeat this procedure with ten ml of unionized water. With column attached, run ml of Cool-Aid solution through it and collect it in beaker. After recording color, run 5 percent of sopranos through syringe and record the color that comes through it. Record color and repeat with 10 ml of percent spoilsport alcohol. Record the color. In order to remove the remaining color, run 70 percent of sopranos through it. The second part of the experiment is spectroscopy.

This was indicated by the data we collected after putting the extracts in the spectrometer with the different wavelengths. It can be more clearly seen in the graph however. The graph shows that the first extract was successfully separated from the Cool-Aid solution, the linear graphs for both of them show very different results. However for the second extract, the line is a lot more similar to the Cool-Aid solution which shows that it may to have separated as well as the first extract. However this is only seen in the 640-700 nanometer range of the graph.

Before that, the components seem to have been separated decently. The colors absorbed by the solution relate to the colors of dye components and mixtures observed by showing the complement of the original color. The color absorbed is the exact opposite of the color originally shown by the color of dye components and mixtures. For example, the color originally absorbed (the color of the Cool-Aid), was a blue violet. What was observed was its complement, which was yellow. This is how the color observed and absorbed relates.

The components of Cool-Aid separated at different steps of the experiment because of the different polarity. Different components were absorbed at different rates throughout the lab. The different kinds of chemicals used also factored in on how the components separated at different rates of the experiment. What determines the polarity is if the components of the solutions were more attracted to liquid or solid parts. Depending on what the preference is, this can cause them to move at different speeds and therefore separate at different steps in the lab.

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Chromatography Lab Report Assignment. (2020, Oct 27). Retrieved August 3, 2021, from