Bio100 Appendix G Assignment

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Interpreting Your Data Plots Although basic trends in your data can sometimes be estimated by simply looking at the data points on your scatter plots, quantitative measures of the effects you are studying can only be determined by fitting a curve to your data. Curve fitting involves producing a statistically derived best-fit line of data points on the graph; not a hand-drawn or estimated line connecting data points. Once you have plotted your data, a Plot # tab will appear at the top of the Plot Data screen.

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The experiment you just performed is representative of other experiments that you will conduct. A lot of information can be learned from studying the curves that you generate. Study the curve of Photosynthetic Rate vs. Light in Tomato to answer the questions below. The following is a representative plot and table from this experiment: SeriesInterceptSlopeAsymptoteError Sum of Squares 1-1. 30. 03817. 10. 198 1. Answer the following questions: •What is the relationship between an increase in light intensity and photosynthetic rate in tomato leaves? Does this relationship support the hypothesis that you formulated? An increase in light intensity increases photosynthetic rate, supporting the hypothesis. •Photosynthetic saturation is the maximum rate of photosynthesis. What was the value for photosynthetic saturation in tomato leaves? •What value of light intensity produced photosynthetic saturation in tomato leaves? •Based on what you know about photosynthesis, provide possible reasons for what causes photosynthetic saturation (these cannot be determined from the plot).

Photosynthetic saturation in tomato leaves will occur at a light intensity of approximately 1600 mol/m2/s. At saturation, photosynthetic rate is approximately 15-17 (as indicated by the asymptote). Exploration Experiment: Light Intensity and Photosynthetic Rates in Corn 1. Follow the steps detailed in the first experiment to test the effects of an increase in light intensity on photosynthetic rates in corn (a C4 plant). •The only modification to the experiment is that you will need to use a high rate of gas flow. Keep all other parameters the same as you did for tomato. 2.

When calculating P and plotting your data, make sure that you select only those values that you recorded for corn and not previously recorded values for tomato. 3. Plot photosynthetic rate versus light intensity and fit a curve to the data as you did for tomato. 4. Add your graph to Appendix H: LeafLab Report by clicking on Export Graph. 5. Copy and paste your graph under the Data section of Appendix H: LeafLab Report. 6. Answer the following questions in Appendix H: LeafLab Report: •What is the relationship between an increase in light intensity and photosynthetic rate in leaves from a corn plant?

How does this relationship compare with what you observed for tomato plants? •Photosynthetic saturation is the maximum rate of photosynthesis. What value of light intensity produced photosynthetic saturation in corn leaves? References LeafLab assignments and answers were adapted with permission from Pearson Education, Inc. Biology Labs On-Line is a collaboration between the California State University system and Benjamin Cummings. © 2002 California State University and Benjamin Cummings, an imprint of Pearson Education, Inc. Development was partially supported by a grant from the U. S. National Science Foundation.

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