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Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorted substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge, alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and separation of sutures of chemical substances.
Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium, in the case of this experiment, filter paper. It is used for the purification and isolation of various substances. A cryptographically pure substance is the result of the separation. Because purification of substances is required to determine their properties, chromatography is an indispensable tool in the sciences concerned with homicidal substances and their reactions.
Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorted substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge, alterations or changes can be detected in the substance. 1. Cut the coffee filter into a rectangle measuring three CM by nine CM. You will need two of these strips. 2.
Draw a line en CM (1/2 inch) from the edge of one end of one strip of paper. 3. Make four pencil dots along this line 0. 5 CM apart. Underneath each dot, label the marker that will be tested. Use the markers to draw a different colored dot on each of the pencil marks on the paper strip. Allow the ink to dry, and then add more color to each dot. Make a prediction of the pigment components you expect to see from each marker’s ink. 4. Mix 1/8 teaspoon of salt and three cups of water in a pitcher or 2-liter bottle.
Shake the solution until it is dissolved. 5. Pour the salt solution into a clean tall glass so that the liquid level is 1/4 inch. 6. Tape the strip to a pencil and rest the pencil on top of the jar so that the strip hangs into the jar. 8. When the salt water is 1/4 inch (0. 5 CM) from the top edge of the paper, remove it from the glass and place it on a clean, flat surface to dry. 9. Repeat steps two through nine to test the same four colors using the alcohol instead of the saltwater solution at the bottom of the glass.