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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a form of energy and would increase the reaction and speed up the reaction time.
It would also cause the starch to hydrolyze before cooler temperatures. Method The substrate that was picked was potato as a starch. We took 10 test tubes and put 3 ml of starch in 5 test tubes. The other 5 test tubes we put 3ml of amylase and pre-incubated them in different temperatures for 5 minutes each. The temperatures that we were using to pre -incubate were: 0, 23, 37, 70 and 100 degrees Celsius. After five minutes we mixed the starch and the amylase and put them into the same bath for 20 minutes.
The next step was to put 1 ml of acid ethanol and add 3 drops of it to perform the IKI test to determine if starch was still present, In which case it would not have been hydrolysised. The variable in this experiment is the temperature. Results of Enzyme Experiment % Transmission Group 0 C 23 C 37 C 70 C 100 C control (+) control (-) mr &tk 0. 0 48. 6 57. 1 0. 0 0. 0 0. 0 100. 0 Jm &Lr 0. 0 64. 1 78. 9 0. 0 0. 0 0. 0 100. 0 JB&JM 0. 0 0. 2 26. 7 0. 0 0. 0 0. 0 100. 0 VR&AT 0. 0 18. 0 38. 0 0. 0 0. 0 0. 0 100. 0 HE&DC 0. 0 70. 9 19. 8 0. 2 0. 0. 0 100. 0 MK&JV&NR 0. 0 39. 5 38. 1 0. 0 0. 0 0. 0 100. 0 NC&KM&JR 0. 0 54. 7 56. 9 0. 0 0. 0 0. 0 100. 0 LG & MA 0. 0 50. 5 56. 2 0. 0 0. 0 0. 0 100. 0 Average 0. 0 43. 3 46. 5 0. 0 0. 0 0. 0 100. 0 Discussion and Conclusion The experiment showed that the temperature does affect a starch. The warmer temperatures did not hydrolyze like the 23 degree and 37 degree temperatures did. 0 degree also did not hydrolyze. Our hypothesis that we listed did not work out like we expected. The higher the temperature the faster the rate of hydrolysis